Predictors of Long-term Patient-Reported Outcome Measures After Collagen Meniscal Implant for Partial Meniscal Defects

Background: Collagen meniscal implant (CMI) is considered an effective procedure for reducing knee pain and improving knee function after previous meniscectomy. Nevertheless, the current knowledge regarding long-term patient reported-outcome measures after CMI is limited. Purpose: To evaluate clinical outcomes, reoperations, and failures of CMI at a minimum 10-year follow-up. Study Design: Case series; Level of evidence, 4. Methods: Consecutive patients who underwent CMI at a single institution were screened for eligibility. Inclusion criteria for the present study were (1) medial or lateral CMI; (2) isolated or combined procedure with anterior cruciate ligament reconstruction, knee osteotomy, or cartilage treatment; and (3) follow-up between 10 and 15 years. Demographics and surgical details were obtained via chart review. Patients were asked if they were satisfied with the procedure and were evaluated with the Lysholm score, Knee injury and Osteoarthritis Outcome Score (KOOS), visual analog scale for pain, and Tegner score at the final follow-up. Cases requiring partial or total scaffold removal for any reason (including scaffold breakage, infection, or surgery for osteoarthritis progression) were considered surgical failure. Survival analysis was performed with Kaplan-Meier curve, and clinical scores were analyzed based on the Patient Acceptable Symptom State (PASS). Results: A total of 92 patients (mean age, 42.2 years were included in the analysis. A significant improvement in all clinical scores was reported between the preoperative evaluation and the last follow-up. A chondropathy with Outerbridge grade ≥3 was associated with significantly overall lower clinical scores, while a timing from meniscectomy to CMI of ≥5 years determined more pain at rest and reduced Quality of Life in the KOOS subscale. No significant difference was found in terms of clinical scores between patients undergoing isolated and combined procedures. At the final follow-up, the mean Lysholm score was 76.3 points. In total, 12 cases (13%) were considered surgical failures. Sixteen patients (17%) did not reach PASS for the Lysholm score, with a total of 28 cases (30%) classified as clinical failures. Overall, 19% (KOOS Pain) and 40% (KOOS Symptoms) of patients did not achieve the PASS in the KOOS subscales. Chondropathy with Outerbridge grade ≥3 was associated with a higher risk of not achieving the PASS in all the KOOS subscales, while age at surgery of ≥45 years resulted in a lower risk of not achieving PASS in the Pain subscale. At the last follow-up, 63% of patients were still involved in sports activity, with 41% at the same or higher level. Finally, 80% of the patients were satisfied with the procedure. Conclusion: Up to 10 years after surgery, around 70% of the patients who underwent CMI reported satisfactory clinical results, with clinical subjective scores still higher compared with the preoperative evaluation. Overall, 30% of cases were considered clinical failures, with 13% considered surgical failures and 17% not meeting the PASS for the Lysholm score. In addition, cartilage status and time from meniscectomy were shown to have a negative impact on the outcomes, while an age ≥45 years was associated with less pain. There was no clinical difference between patients who underwent isolated CMI or combined procedures.

Menisci play an essential role in knee biomechanics and are crucial for articular cartilage preservation. 1Research has shown that even a partial meniscectomy can lead to The Orthopaedic Journal of Sports Medicine, 12 (7), 23259671241254395 DOI: 10.1177/23259671241254395 Ó The Author(s) 2024 unicompartmental knee pain, recurrent swelling, and bone marrow edema in certain patients, potentially resulting in early onset osteoarthritis. 4This condition is also known as ''postmeniscectomy syndrome.''To avoid and manage this condition, meniscal scaffold devices have been developed and proposed as an alternative to meniscal allograft transplantation (MAT) only in the case of partial meniscal loss. 4,5ven though it has been .20 years since the first reports on the use of meniscal scaffolds, limited literature exists on patient and surgical factors that may affect the long-term clinical outcomes of this procedure since most of the scaffold studies are limited to a short-or midterm follow-up.The few studies that evaluated the long-term outcomes of this procedure assessed mostly the failure rate and had a small sample size. 11,18,27oreover, a recent systematic review evaluating the outcomes of meniscal scaffold implantation concluded that due to the presence of overlapping patients and frequent associated concomitant surgeries, most of the clinical benefit might be related to these concomitant procedures rather than the partial meniscal substitution procedures. 13gainst this background, the primary purpose of this study was to assess the long-term clinical outcomes of the collagen meniscal implant (CMI) for partial meniscal defect, investigating if comparable results can be obtained after an isolated procedure or in combination with anterior cruciate ligament (ACL) reconstruction, knee osteotomy, or cartilage treatment.The secondary purpose was to identify possible predictors of clinical outcomes.The hypothesis was that good clinical outcomes could be obtained with CMI, both in isolated cases and in combination with other treatments.Additionally, patients with more severe chondral damage would have poorer outcomes, and delaying scaffold implantation after meniscectomy could affect clinical results.

Patient Selection
Institutional review board approval was obtained for the study (General protocol number: 0014969 -02/11/2020).No specific funding was provided for the present study.All the consecutive patients who underwent CMI scaffold implantation at Rizzoli Orthopaedic Institute between 2005 and 2010 were screened.The indications for scaffold implantation and the study inclusion criteria are shown in Table 1.All the patients signed an informed consent.

Patient Evaluation
Demographics and surgical details such as patient sex, CMI side (medial or lateral), body mass index (BMI), time from first meniscectomy to CMI, concurrent procedures, and cartilage status were obtained by chart review.Patients with acute irreparable tears who underwent meniscectomy and immediate CMI implantation were considered to have a time from meniscectomy to CMI of 0 years.For the purpose of the analysis, the chondropathy was stratified as ''low grade'' in the case of Outerbridge grades 0 to 2 and ''high grade'' in the case of Outerbridge grades 3 and 4.
All patients were contacted via telephone, and Knee injury and Osteoarthritis Outcome Score (KOOS), Lysholm, and Tegner questionnaires were administered.Patients were also asked to rate their knee pain at rest or during activity on a 10-point visual analog scale (VAS).Data on sport activity and participation were also investigated.Patients were asked whether they had undergone any additional surgeries on the operated knee during the follow-up period and if they were satisfied with the results of the CMI.Finally, medical records from the hospital database were screened for possible complications or further surgeries.

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Surgical Technique and Rehabilitation
The surgical technique for arthroscopic CMI implantation has been previously described. 11,27Briefly, a standard diagnostic arthroscopy was performed to confirm the indication of CMI; the ACL should be intact or concomitantly reconstructed.While diffuse grade 4 Outerbridge cartilage degeneration represented a contraindication to scaffold implant, it was treated with standard cartilage procedures such as microfracture, mosaicplasty, or scaffold implants if a focal full-thickness cartilage lesion was present.The CMI for the partial meniscal defect was implanted arthroscopically and sutured to the meniscal remnant with all-inside devices.Then, any associated procedures such as an ACL reconstruction, osteotomy, or cartilage repair surgery were performed.
A knee brace locked in full extension was applied for 6 weeks.To avoid knee stiffness, continuous passive motion was performed 4 times per day, from 0°to 60°during the first 2 weeks.The range of motion then increased to 90°f rom the second to the sixth week, while complete range of motion was allowed starting from the sixth week.Weightbearing was avoided for the first 3 weeks.After this period, 30% of the patient's weightbearing was allowed during the fourth week and 50% of the patient's weightbearing was allowed during the fifth week, with progression to full weightbearing during the sixth week.Return to sports and cutting activity was permitted starting 6 months after surgery. 27

Statistical Analysis
Statistical analysis was performed with MedCalc (Acacialaan 22; MedCalc software).The continuous variables were expressed as mean 6 SD, while the categorical variables were expressed as numbers and percentages.A comparison among various follow-up points and between the isolated and combined groups was performed with unpaired-samples t test in case of continuous variables, and differences in categorical variables were analyzed with the chi-square test.The patients were also stratified according to the PASS threshold for the Lysholm score (66.5 points), KOOS Symptoms (73.0 points), KOOS Pain (43.0 points), KOOS Activities of Daily Living (KOOS ADL) (74.5 points), KOOS Sport and Recreation (KOOS Sport) (22.5 points) and KOOS Quality of Life (KOOS QoL) (53.0 points) as defined by Liu et al 15 for meniscal transplantation.In the case of missing values for clinical scores owing to surgical failure, the preoperative values were used to avoid overestimation of the clinical status when calculating the postoperative mean values.Moreover, patients with surgical failure were considered as not having achieved the PASS for all the scores and subscales.The total of patients with surgical failure and those not reaching the PASS for the Lysholm score were defined as having experienced clinical failure.Survival analysis was performed with Kaplan-Meier curve.
Multiple regression analysis models with a stepwise approach were performed using clinical scores as endpoints and sex (male vs female), CMI side (medial vs lateral), age (\45 years vs 45 years), 3 chondropathy (Outerbridge grades 0-2 vs 3-4), associated procedures (isolated vs combined), BMI (\25 kg/m 2 vs 25 kg/m 2 ), 28 and time from meniscectomy (\5 years vs 5 years) 17 as independent variables.The same variables were used in logistic regression analysis models when assessing patients not achieving the PASS for the various scores; the results were reported as odd ratios with 95% CI.Multiple and logistic regression analyses were normalized for the preoperative value of each score.Differences were considered significant with P \ .05.

Patient Characteristics
A total of 111 patients met the inclusion criteria, and 92 (83%) patients completed the long-term assessment at a mean follow-up of 11.8 6 1.8 years (Figure 1).In the study population, there were 70 men (76%) and 22 women (24%) with a mean age at surgery of 42.2 6 10.8 years.A total of 68 patients received medial CMI (74%) while 24 received lateral CMI (26%) after a mean of 9.7 6 8.9 years from the meniscectomy (Table 2).A total of 36 cases (39%) were ''isolated'' while 56 cases (61%) were ''combined'' procedures.In the study population, 19 patients (21%) underwent acute CMI implantation (within 6 months from the previous meniscectomy) and 14 (15%) patients underwent CMI as an isolated procedure.There were 24 patients (26%) who underwent an associated cartilage treatment (Table 2).Twelve cases were located on the medial femoral condyle, 4 on the medial tibial plateau, 6 on the lateral femoral condyle, 1 on the lateral tibial plateau, and 1 on the trochlea.The cartilage lesions were 1 to 2 cm 2 in 6 patients, 2 to 3 cm 2 in 10 patients, and .3cm 2 in 8 patients.

Clinical Outcomes
A total of 25 patients (27%) underwent a surgical procedure during the considered follow-up, at a mean of 4.3 6 4.0 years from the CMI.Of these, only 12 cases (13%) were considered surgical failures (Table 3).Moreover, 19% of patients did not achieve the PASS for the KOOS Pain subscale, 40% for the KOOS Symptoms subscale, 23% for the KOOS ADL subscale, 24% for the KOOS Sport subscale, and 36% for the KOOS QoL subscale.

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A significant improvement of the Lyholm score was reported from 55.6 6 17.2 preoperatively to 76.3 6 19.6 at final follow-up (P = .0001).Overall, 17% of patients did not achieve the PASS for Lysholm.Considering surgical failures and patients who did not reach the PASS for Lysholm score, a total of 28 cases (30%) were considered clinical failures.
A total of 61% of patients were still practicing sports at the final follow-up, with 41% at the same or higher level.In particular, 34 patients were involved in pivoting sports (such as basketball, soccer, tennis, skiing), 8 patients were involved in hiking or trekking activities, 7 patients were still swimming, 3 patients were cycling, 1 patient was still involved in triathlon and 3 patients were practicing other sports.The overall mean satisfaction was 4.0 out of 5.0, and 80% of patients were satisfied by the procedure.

Isolated Versus Combined CMI
Demographics and surgical characteristics were similar between the patients with isolated CMI (n = 36) and those with a combined procedure (56 patients), except for the degree of chondropathy and the time since meniscectomy, which were significantly higher in the case of combined procedures (Table 4).
Regarding clinical outcomes, significant improvements of all patient-reported outcome measures were reported in both isolated and combined groups (Table 5).No significant differences were seen in patient-reported outcome   measure assessment between the 2 groups, and a higher postoperative proportion of patients achieved PASS for the KOOS Pain subscale in the isolated group at the follow-up (Figure 4).
A significantly higher proportion of reoperations was reported in the combined group (36%) with respect to the isolated group (14%); however, no difference between the 2 groups was reported for clinical and surgical failures despite higher rates reported in the case of a combined procedure (Table 5).

Assessment of Outcome Predictors
According to the multiple regression analysis performed on the whole series, having a chondropathy of Outerbridge grades 3 to 4 resulted in -16.5 points in the KOOS Pain subscale, -11.3 points in the KOOS Symptoms subscale, -7.0 points in the KOOS ADL subscale, -15.6 points in the KOOS Sport subscale, -8.4 points in the Lysholm score, 1 1.4 points in VAS pain at rest and 1 2.6 points in VAS pain during activity (Table 6); moreover, significantly lower values were reported in patients with chondropathy grades 3 to 4 compared with those with chondropathy grades 0 to 2 (Figure 5).Another outcome predictor was the timing of CMI of 5 years after the meniscectomy, which resulted in 1 0.9 points in VAS pain at rest and -12.3 points in the KOOS QoL subscale.

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Deleterious Effect of Cartilage Damage 5 According to the logistic regression analysis, having a chondropathy of Outerbridge grades 3 to 4 resulted in a higher risk of not achieving the PASS for all the KOOS subscales and Lysholm, while an age at surgery of 45 years resulted in a lower risk of not achieving PASS for the KOOS Pain subscale (Table 7).

DISCUSSION
The most important finding of the present study is that 70% of patients achieved satisfactory clinical results, whereas 13% of cases were surgical failures and another 17% had intact implants but poor clinical scores.The sports activity level steadily improved compared with the preoperative status, and almost two-thirds of the patients were still active at the long-term evaluation.Furthermore, several factors were associated with lower clinical scores, including an increasing time from meniscectomy to scaffold implantation, age, and Outerbridge grade.
Satisfactory clinical outcomes of the meniscal scaffold implantation have been previously reported, with an improvement in symptoms and function compared with the preoperative status at short-to midterm follow-up. 20owever, the authors reported that only 3 case series investigated the long-term outcomes of CMI, and none of the cases evaluated the PASS and the predictive factors affecting the clinical outcomes after meniscal scaffold substitution.
Two long-term case series with \40 patients reported significant and stable clinical outcomes at a 10-year follow-up after medial meniscal scaffold implantation with only 2 overall failures. 18,27On the other hand, results from a long-term multicenter study on the lateral CMI reported a significantly higher failure rate, more pain, and a lower activity level when compared with the results in existing literature on the medial scaffold. 11Similarly, a recent long-term survival analysis identified the lateral scaffold as a risk factor for surgical failure, with a 10year survival rate of 77% versus 90% of the medial CMI. 17 Conversely, in the present study, the multivariate analysis found no significant difference in patient-reported outcome measures concerning the medial versus lateral  scaffold.Those results align with a recent CMI and polyurethane meniscal scaffold meta-analysis that found no differences in clinical outcomes or survival rates between medial and lateral meniscal scaffolds at short-or midterm follow-up. 19Moreover, 70% of the patients achieved the PASS for Lysholm score; this could be considered a satisfactory result if we consider that meniscal substitution is usually performed on patients affected by postmeniscectomy syndrome and limited alternative procedures to joint replacement.The results reported in the present study are in line with a clinical study investigating the outcomes of MAT at a similar follow-up time, with a Lysholm score around 80 points at 10-year follow-up. 10n the current study, the return-to-sports rate, reduced pain during activity, and Tegner level at the final follow-up should be considered satisfactory considering the mean age of the patients and the multiple knee comorbidities of the study population.Similar to studies investigating the outcomes of MAT, return-to-sports activity appears to be a reasonable expectation after meniscal scaffold substitution, especially for low-impact sports. 9owever, because there are few studies and limited evidence about the impact of sports activity and cartilage degeneration after meniscal substitution, high-impact activities must be considered with caution, keeping in mind that the general ambition should be to preserve the knee function for ADLs and work. 20,21,23,24n interesting finding of the present study is that patients who underwent isolated CMI showed similar overall outcomes but less knee pain when compared with patients who underwent combined surgeries.These results contrast with those of Gelber et al, 7 who reported inferior clinical results in patients with varus knee and meniscal deficiency when the high tibial osteotomy was associated with the implant of a polyurethane meniscal scaffold.
Our findings further underline the effect of the CMI as a standalone procedure and contest the conclusion of a recent systematic review which hypothesized that the presence of concomitant surgeries may have a significant influence on the positive results after scaffold substitution. 13However, considering that 14 out of 36 patients (39%) included in the isolated group underwent acute CMI implantation, it could also be hypothesized that the clinical benefit could be related to the meniscectomy itself rather than the scaffold.For this reason and due to the heterogeneity of the study population, it remains challenging to draw a definitive conclusion regarding the effect of the isolated CMI.
In the present study, sex and BMI did not show any impact on the clinical outcomes.However, while the effect of sex has been less investigated in the meniscal scaffold literature, 2 separate reports showed a higher incidence of revision and surgical failures in female patients requiring MAT. 6,10Moreover, in contrast to our results, a higher BMI was correlated with inferior clinical results in a multicentric study of 43 patients who underwent lateral CMI. 26 Thus, the   effect of these 2 parameters on the clinical outcomes should be further investigated due to inconclusive evidence.One of the most debated topics in the meniscal substitution literature is the ideal time elapse between meniscectomy and meniscal transplant or scaffold implant. 8In the present study, we found that patients who waited 5 years for meniscal scaffold substitution reported significantly higher pain scores and reduced QoL at the final follow-up.
In a large case series of CMI, the authors reported that waiting .10 years after meniscectomy was correlated with a significantly higher risk of surgical or clinical failure regardless of the cartilage status. 16Another study reported that waiting for as little as 6 months after a meniscectomy may reduce clinical outcomes and sports level at shortterm follow-up. 2Finally, a study on the effect of meniscal transplant found that an acute MAT achieved better cartilage and meniscal protection in the long term compared with the conventional delayed MAT.However, the impact on clinical outcomes was minimal. 25Although some of the studies mentioned above found better clinical outcomes if a meniscal substitution procedure was performed in an acute setting, little is known about risk factors for developing postmeniscectomy syndrome.Therefore, further research is needed to help the surgeon identify the patients who may benefit from an acute scaffold or MAT surgery.
Another interesting result of the present study was that patients 45 years old not only showed similar outcomes when compared with younger patients but were also more likely to achieve the PASS in the KOOS Pain subscale.A previous study showed that age was not correlated with surgical failure. 22The present study's clinical results further confirm that age does not represent a contraindication for meniscal scaffold.These data appear promising, considering that one of the most common patient profiles of the candidates for meniscal scaffold is represented by a middle-aged man with a previous partial meniscectomy or with meniscal tears on degenerated tissue.During scaffold substitution, the patient should be carefully evaluated.Clinical outcomes and survivorship are predominantly affected by age-related factors, including time from meniscectomy and chondropathy, rather than age itself. 22This is similar to the MAT.
Despite the strength of a long-term minimum follow-up of 10 years, the large number of patients, and the satisfactory follow-up rate, the present study presents several limitations.First, this is a retrospective study that lacks a control group of patients who underwent isolated meniscectomy or meniscal transplant.Moreover, the patients are heterogeneous in terms of age, sex, chondropathy, and concomitant procedures, making it more difficult to predict the outcomes of a specific population.However, the presence of multiple knee comorbidities represents a typical scenario of patients referred to a tertiary sports medicine center.Therefore, multiple subanalyses were performed to evaluate each parameter individually.Additionally, the PASS used in the present analysis was validated for patients requiring MAT. 15 Nevertheless, it is to be considered that there are no PASS scores validated for meniscal scaffold and that the demographic characteristics and knee pathology of patients requiring 1 of either of these 2 procedures are similar.Last, since no magnetic resonance imaging was performed at the last follow-up, it was not possible to evaluate parameters such as cartilage degeneration over time, scaffold extrusion, or reabsorption.However, this study was specifically designed to evaluate predictors of clinical outcomes and the radiological classification system.[14] CONCLUSION Up to 10 years after surgery, around 70% of the patients who underwent CMI reported satisfactory clinical results, with subjective scores still higher compared with the preoperative evaluation.A total of 28 cases (30%) were classified as clinical failures (13% surgical failure and 17% not meeting PASS for Lysholm score).In addition, the cartilage status and the time from meniscectomy were shown to have a negative impact on outcomes, while an age 45 years was associated with less pain.Finally, there was no clinical difference between patients who underwent isolated CMI or combined procedures.

Figure 2 .
Figure 2. Comparison of pain at rest and pain during activity preoperatively and postoperatively.*Statistically significant at P \ .005.VAS, visual analog scale.

Figure 3 .
Figure 3. Knee injury and Osteoarthritis Outcome Score (KOOS) subscales preoperatively and postoperatively.*Statistically significant at P \ .005.ADL, Activities of Daily Living; QoL, Quality of Life; Sport, Sport and Recreation.

Figure 4 .
Figure 4.A comparison of isolated and combined CMI based on the KOOS subscale.ADL, Activities of Daily Living; CMI, collagen meniscal implant; KOOS, Knee injury and Osteoarthritis Outcome Score; QoL, Quality of Life; Sport, Sport and Recreation.

a
Multivariate analysis of the effect on patient-reported outcomes of patients and surgical characteristics.Significative p values are in bold (p .0.05) ADL, Activities of Daily Living; BMI, body mass index; CMI, collagen meniscal implant; KOOS, Knee injury and Osteoarthritis Outcome Score; N.S., nonsignificant; QoL, Quality of Life; Sport, Sport and Recreation; VAS, visual analog scale.

Figure 5 .
Figure 5.Comparison of Outerbridge grades 0 to 2 and grades 3 to 4 according to cartilage status.*Statistically significant difference between the groups (P \ .05).ADL, Activities of Daily Living; KOOS, Knee injury and Osteoarthritis Outcome Score; QoL, Quality of Life; Sport, Sport and Recreation.

TABLE 1
Indications for Surgery and Inclusion Criteria aAddress correspondence to Gian Andrea Lucidi, MD, Clinica Ortopedica e Traumatologica II, IRCCS Istituto Ortopedico Rizzoli, Via C.Pupilli 1, Bologna, 40133, Italy (email: gianandrea.lucidi@studio.unibo.it).Final revision submitted December 1, 2023; accepted December 6, 2023.One or more of the authors has declared the following potential conflict of interest or source of funding: S.Z. is a consultant for Smith+Nephew and DePuy Synthes and has received research support from Medacta and DePuy Synthes.AOSSM checks author disclosures against the Open Payments Database (OPD).AOSSM has not conducted an independent investigation on the OPD and disclaims any liability or responsibility relating thereto.Ethical approval for this study was obtained from the Istituto Ortopedico Rizzoli di Bologna and Istituto di Ricovero e Cura a Carattere Scientifico (ref No. 0014969).

TABLE 2 Patient
Demographics and Surgical Characteristics a (n = 92)

TABLE 4
Demographics of Patients With Isolated CMI Versus CMI With Combined Procedures a aValues are presented as mean 6 SD or n (%), unless otherwise indicated.BMI, body mass index; CMI, collagen meniscal implant; IQR, interquartile range.

TABLE 5
Outcomes of Patients With Isolated CMI Versus CMI With Combined Procedures a

TABLE 6 Multivariate
Analysis of Patient-Reported Outcome Measures a

TABLE 7
Analysis of PASS for KOOS and Lysholm Scores a (n = 92) Significative p values are in bold (p .0.05) ADL, Activities of Daily Living; BMI, body mass index; CMI, collagen meniscal implant; KOOS, Knee injury and Osteoarthritis Outcome Score; N.S., nonsignificant; OR, odds ratio; PASS, Patient Acceptable Symptom State; QoL, Quality of Life; Sport, Sport and Recreation. a